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KMID : 0368419860290040275
Journal of Plant Biology
1986 Volume.29 No. 4 p.275 ~ p.283
Physiologieal Studies on the Farmation of Hairy Root by the Agrobacterium rhizogenes



Abstract
Inoculation of carrot discs with Agrobacterium rhizogenes harboring Ri plasmid resulted in transformation of cells, as revealed by the tumors and hairy roots arising from them. Measurements of IAA content using HPLC indicate that it is higher in tumors and inoculated tissues than in uninoculated tissue. A lot of meristemoids and vessel elements formed in tumor tissue and the hiary roots differentiated from meristemoids. IAA content in tumor tissues is decreased with hairy root and vessel elements formation from them. Formation of wound callus in uninoculated tissues resulted on wound healing but no formation of vessel elements and hairy roots. Tumor tissues show continuous growing on hormone free medium.
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